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Test Code HBEA Hemoglobin Evaluation by HPLC With Reflex to Electrophoresis and/or RBC Solubility

Important Note

Effective test to screen for hemoglobinopathies. May be used for follow up in individuals with a known hemoglobinopathy.

If abnormal peaks suggestive of a hemoglobin variant are detected, then RBC solubility and/or capillary electrophoresis will be performed to aid in confirmation and identification of the variant. Additional charges apply.

If a hemoglobin variant cannot be quantitated by HPLC, results from capillary electrophoresis will be reported.

Quantitation of hemoglobin is recommended for a definitive diagnosis in infants 1 year and older.

Specimen Volume

5 mL

Preferred Specimen:

Whole Blood from a Lavender (EDTA) or Pink (K2EDTA)

Specimen Handling Instructions

Critical Refrigerated

Transport

Transport 5 mL whole blood in original tube

Performing Laboratory

ARUP Laboratories

Methodology

High Performance Liquid Chromatography (HPLC) / Capillary Electrophoresis / RBC Solubility

Routine Turn-around Time

1-5 Days

Reference Interval

Test Number Components Reference Interval
  Hemoglobin A Age                   Reference Intervals (%)
0-1 months                    7.6-54.8
2 months                        14.7-70.1
3 months                        26.6-81.8
4 months                        43.0-89.5
5 months                        60.8-94.0
6-8 months                    78.2-96.6
9-12 months                  86.1-97.2
13-23 months                85.1-97.7
2 years and older           95.0-97.9
  Hemoglobin A2 Age                Reference Intervals (%)
0-1 months                    0.0-1.4
2 months                        0.0-2.0
3 months                         0.1-2.6
4 months                         0.8-3.0
5 months                         1.5-3.3
6-8 months                     1.8-3.5
9-23 months                   1.9-3.5
2 years and older          2.0-3.5
  Hemoglobin F Age                     Reference Intervals (%)
0-1 months                   45.8-91.7
2 months                       32.7-85.2
3 months                       14.5-73.7
4 months                       4.2-56.9
5 months                       1.0-38.1
6-8 months                    0.9-19.4
9-12 months                  0.6-11.6
13-23 months                 0.0-8.5
2 years and older            0.0-2.1
  Hemoglobin S 0.0
  Hemoglobin C 0.0
  Hemoglobin E 0.0
  Hemoglobin Other 0.0

CPT Codes

83021; if reflexed, add 83020; 85660

LOINC Codes

4547-6

4551-8

4576-5

4625-0

4563-3

4575-7

48343-8

21026-0

6864-3

13514-5